Revista Mexicana de Oftalmología

Register      Login

VOLUME 95 , ISSUE 2E ( March-April, 2021 ) > List of Articles

Original Article

Effect of 3% topical trehalose on the rate of corneal re-epithelialization in patients undergoing photorefractive keratectomy

Alberto Haber, Eduardo J. Polanía-Barón

Keywords : Refractive surgical procedures, Photorefractive keratectomy, Re-epithelialization, Trehalose

Citation Information : Haber A, Polanía-Barón EJ. Effect of 3% topical trehalose on the rate of corneal re-epithelialization in patients undergoing photorefractive keratectomy. 2021; 95 (2E):68-72.

DOI: 10.24875/RMOE.M21000158

License: CC BY-NC-ND 4.0

Published Online: 16-03-2021

Copyright Statement:  Copyright © 2020 Sociedad Mexicana de Oftalmología. Published by Permanyer


Abstract

Purpose: To study the changes in corneal re-epithelialization rate in patients undergoing surface photorefractive keratectomy by using 3% trehalose. Methods: This is a prospective, randomized, controlled clinical trial. The inclusion criteria were patients older than 18 years, with clinical and topographic criteria for refractive surgery, who agreed to participate in the protocol. Randomization was carried out to assign one eye of each patient to the control group with conventional treatment and the other eye to the experimental group with 3% trehalose plus conventional treatment. The free image processing software Image J was used to quantify the area of de-epithelialization in square millimeters. Results: The average number of epithelialization days was of 2.984 days in the experimental group and of 2.954 days in the control group (not statistically significant; p = 0.1624063). The area of de-epithelialization in the examination prior to reaching 100% epithelialization was of 4.255 mm2 in the experimental group and 3.1762 mm2 in the control group (not statistically significant; p = 0.0899374). Conclusions: Topical instillation of 3% trehalose does not increase the rate of corneal re-epithelialization in patients undergoing photorefractive keratectomy.


PDF Share
  1. Crowe JH, Hoekstra FA, Crowe LM. Anhydrobiosis. Annu Rev Physiol. 1992;54:579-99.
  2. Cejka C, Kubinova S, Cejkova J. Trehalose in ophthalmology. Histol Histopathol. 2019;34:611-8.
  3. Cejková J, Cejka C, Ardan T, Sirc J, Michálek J, Luyckx J. Reduced UVB induced corneal damage caused by reactive oxygen and nitrogen species and decreased changes in corneal optics after trehalose treatment. Histopathol. 2010;25:140316.
  4. Jain NK, Roy I. Effect of trehalose on protein structure. Protein Sci. 2009;18:24-36.
  5. Guo N, Puhlev I, Brown DR, Mansbridge J, Levine F. Trehalose expression confers desiccation tolerance on human cells. Nat Biotechnol. 2000;18:168.
  6. Matsuo T. Trehalose protects corneal epithelial cells from death by drying. Br J Ophthalmol. 2001;85:610-2.
  7. Hovakimyan M, Ramoth T, Löbler M, Schmitz K, Witt M, Guthoff R, et al. Evaluation of protective effects of trehalose on desiccation of epithelial cells in three dimensional reconstructed human corneal epithelium. Curr Eye Res. 2012;37:982-9.
  8. Aragona P, Colosi P, Rania L, Colosi F, Pisani A, Puzzolo D, et al. Protective effects of trehalose on the corneal epithelial cells. Scientific World Journal. 2014;2014:717835.
  9. Li J, Roubeix C, Wang Y, Shi S, Liu G, Baudouin C, et al. Therapeutic efficacy of trehalose eye drops for treatment of murine dry eye induced by an intelligently controlled environmental system. Mol Vis. 2012;18:317-29.
  10. Chen W, Zhang X, Liu M, Zhang J, Ye Y, Lin Y, et al. Trehalose protects against ocular surface disorders in experimental murine dry eye through suppression of apoptosis. Exp Eye Res. 2009;89:311-8.
  11. Cejková J, Cejka C, Luyckx J. Trehalose treatment accelerates the healing of UVB irradiated corneas. Comparative immunohistochemical studies on corneal cryostat sections and corneal impression cytology. Histol Histopathol. 2012;27:1029-40.
  12. Matsuo T. Trehalose versus hyaluronan or cellulose in eyedrops for the treatment of dry eye. Jpn J Ophthalmol. 2004;48:321-7.
  13. Matsuo T, Tsuchida Y, Morimoto N. Trehalose eye drops in the treatment of dry eye syndrome. Ophthalmology. 2002;109:2024-9.
  14. Ozek D, Kemer O. Effect of the bioprotectant agent trehalose on corneal epithelial healing after corneal cross-linking for keratoconus. Arq Bras Oftalmol. 2018;81:505-9.
  15. Akcam HT, Unlu M, Karaca EE, Yazici H, Aydin B, Hondur AM. Autologous serum eye-drops and enhanced epithelial healing time after photorefractive keratectomy. Clin Exp Optom. 2018;101:34-7.
  16. Marino GK, Santhiago MR, Santhanam A, Torricelli AAM, Wilson SE. Regeneration of defective epithelial basement membrane and restoration of corneal transparency after photorefractive keratectomy. J Refract Surg. 2017;33:337-46.
  17. Taneri S, Oehler S, MacRae S, Dick HB. Infiuence of a therapeutic soft contact lens on epithelial healing, visual recovery, haze, and pain after photorefractive keratectomy. Eye Contact Lens. 2018;44 (Suppl 1):S38-S43.
  18. Ilhan A. Autologous serum eye drops accelerate epithelial healing after LASEK. Curr Eye Res. 2016;41:872.
  19. Serrao S, Lombardo M. Corneal epithelial healing after photorefractive keratectomy: analytical study. J Cataract Refract Surg. 2005;31:930-7.
  20. Verma S, Corbett MC, Marshall J. A prospective, randomized, double-masked trial to evaluate the role of topical anesthetics in controlling pain after photorefractive keratectomy. Ophthalmology. 1995;102:1918-24.
  21. Zarei-Ghanavati S, Jafarpour S, Radyn-Majd A, Hosseinikhah-Manshadi H. Evaluation of early postoperative ocular pain after photorefractive keratectomy and corneal crosslinking. J Cataract Refract Surg. 2018;44: 566-70.
  22. Hill-Bator A, Misiuk-Hojto M, Marycz K, Grzesiak J. Trehalose-based eye drops preserve viability and functionality of cultured human corneal epithelial cells during desiccation. Biomed Res Int. 2014;2014:292139.
  23. Mateo Orobia AJ, Casas Pascual P, Cristóbal Bescós JA, Pérez García D, Peiro Embid C, Del Buey Sayas MA, et al. Effects of 3% trehalose as an adjuvant treatment after LASIK. Clin Ophthalmol. 2017;11:347-53.
  24. Marshall J, Trokel SL, Rothery S, Krueger RR. Long-term healing of the central cornea after photorefractive keratectomy using an excimer laser. Ophthalmology. 1988;95:1411-21.
  25. Kinoshita JH. Physiological chemistry of the eye. Arch Ophthalmol. 1962;68:554-70.
  26. Gandhi S, Jain S. The anatomy and physiology of cornea. Keratoprostheses Artif Corneas Fundam Surg Appl. 2015;37:19-25.
PDF Share
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.